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To compare anion amount between polluted and unpolluted areas the amount of anion was counted in forest, urban district, and industrial area of urban district. Factors related to anion evolution in plant were analyzed using Populus sieboldii M., Pinus densiflora S., Glochidion obovatum S. It was found that anion was lower in the urban area than in forest; it showed that plants were significantly involved in anion evolution. In this case, light and humidity, among various factors related to anion evolution in plants, had influence mainly on the generation of anion directly since the generation of anion augments with the increase of light intensity and humidity. It suggests that humidity raised by transpiration acts as a major factor to the anion evolution in plant. The anion evolution by plant species was in the order of P. sieboldii, G. obovatum, and P. densiflora, and this result was concomitant with differences of transpiration. We found that plants showing active transpiration may be used for maximizing the effect of anion evolution.
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